Laser pyrolysis products: sampling procedures, cytotoxic and genotoxic effects
Identifieur interne : 000C29 ( Main/Exploration ); précédent : 000C28; suivant : 000C30Laser pyrolysis products: sampling procedures, cytotoxic and genotoxic effects
Auteurs : Bernd Stocker [Allemagne] ; Thomas Meier [Allemagne] ; Theodor M. Fliedner [Allemagne] ; Ulla Plappert [Allemagne]Source :
- Mutation Research - Genetic Toxicology and Environmental Mutagenesis [ 1383-5718 ] ; 1997.
Abstract
The use of lasers in medical applications has grown enormously in the last few years. Recent chemical analysis of the laser pyrolysis products revealed that aerosols generated by pyrolytic decomposition of tissue could be health hazards. Therefore we analysed the genotoxic and mutagenic effects of laser pyrolysis products form different types of porcine tissue. The tissues were irradiated with a surgical CO2 laser and the generated aerosols were sampled as particulate fractions as well as low and highly volatile fractions. Then human leukocytes were incubated with the pyrolysis products and subjected to the comet assay. The results of the comet assay indicated the pyrolysis products being inducers of DNA damage. The ability to induce genotoxic effects turned out to be strongly dependent on the type of tissue that had been irradiated during laser treatment. To check whether the pyrolysis products also have mutagenic properties the Salmonella mutagenicity assay was performed. The particulate aerosol fractions of skin, muscle tissue and liver tissue clearly proved to be mutagenic in TA98 in the presence of S9 mix. There was no mutagenic effect detectable without metabolic activation. In conclusion, our experiments showed that the laser pyrolysis products originating from porcine tissues induced very potent genotoxic as well as mutagenic effects and therefore they could be potential health hazards for humans.
Url:
DOI: 10.1016/S1383-5718(97)00182-4
Affiliations:
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Fliedner</name></author><author><name sortKey="Plappert, Ulla" sort="Plappert, Ulla" uniqKey="Plappert U" first="Ulla" last="Plappert">Ulla Plappert</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:63EEBAD444C7A01026C548F9417A8C859F2D9444</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1016/S1383-5718(97)00182-4</idno><idno type="url">https://api.istex.fr/document/63EEBAD444C7A01026C548F9417A8C859F2D9444/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000668</idno><idno type="wicri:Area/Main/Curation">000664</idno><idno type="wicri:Area/Main/Exploration">000C29</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Exploration">000C29</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Laser pyrolysis products: sampling procedures, cytotoxic and genotoxic effects</title><author><name sortKey="Stocker, Bernd" sort="Stocker, Bernd" uniqKey="Stocker B" first="Bernd" last="Stocker">Bernd Stocker</name><affiliation wicri:level="4"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Clinical Physiology, Occupational and Social Medicine, University of Ulm, Albert-Einstein Allee 11, D 89070 Ulm</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region><settlement type="city">Ulm</settlement></placeName><orgName type="university">Université d'Ulm</orgName></affiliation></author><author><name sortKey="Meier, Thomas" sort="Meier, Thomas" uniqKey="Meier T" first="Thomas" last="Meier">Thomas Meier</name><affiliation wicri:level="4"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Lasertechnologien in der Medizin und Meßtechnik, University of Ulm, Albert-Einstein Allee 11, D 89070 Ulm</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region><settlement type="city">Ulm</settlement></placeName><orgName type="university">Université d'Ulm</orgName></affiliation></author><author><name sortKey="Fliedner, Theodor M" sort="Fliedner, Theodor M" uniqKey="Fliedner T" first="Theodor M" last="Fliedner">Theodor M. 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Recent chemical analysis of the laser pyrolysis products revealed that aerosols generated by pyrolytic decomposition of tissue could be health hazards. Therefore we analysed the genotoxic and mutagenic effects of laser pyrolysis products form different types of porcine tissue. The tissues were irradiated with a surgical CO2 laser and the generated aerosols were sampled as particulate fractions as well as low and highly volatile fractions. Then human leukocytes were incubated with the pyrolysis products and subjected to the comet assay. The results of the comet assay indicated the pyrolysis products being inducers of DNA damage. The ability to induce genotoxic effects turned out to be strongly dependent on the type of tissue that had been irradiated during laser treatment. To check whether the pyrolysis products also have mutagenic properties the Salmonella mutagenicity assay was performed. The particulate aerosol fractions of skin, muscle tissue and liver tissue clearly proved to be mutagenic in TA98 in the presence of S9 mix. There was no mutagenic effect detectable without metabolic activation. In conclusion, our experiments showed that the laser pyrolysis products originating from porcine tissues induced very potent genotoxic as well as mutagenic effects and therefore they could be potential health hazards for humans.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Tübingen</li></region><settlement><li>Ulm</li></settlement><orgName><li>Université d'Ulm</li></orgName></list><tree><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Stocker, Bernd" sort="Stocker, Bernd" uniqKey="Stocker B" first="Bernd" last="Stocker">Bernd Stocker</name></region><name sortKey="Fliedner, Theodor M" sort="Fliedner, Theodor M" uniqKey="Fliedner T" first="Theodor M" last="Fliedner">Theodor M. Fliedner</name><name sortKey="Meier, Thomas" sort="Meier, Thomas" uniqKey="Meier T" first="Thomas" last="Meier">Thomas Meier</name><name sortKey="Plappert, Ulla" sort="Plappert, Ulla" uniqKey="Plappert U" first="Ulla" last="Plappert">Ulla Plappert</name><name sortKey="Plappert, Ulla" sort="Plappert, Ulla" uniqKey="Plappert U" first="Ulla" last="Plappert">Ulla Plappert</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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